![]() ![]() Genotype data was compared and evaluated using three population genetics software. The settlements are divided into 133 sub-tribes. They are settled in the western part of India, which includes the states of Gujarat and Rajasthan. ‘Rabari’, also known as Rewari or Desai, derived from the word Sanskrit, means ‘outsiders’ (Kohler-Rollefson 1992). Here, we have reported allele frequencies and forensic parameters of 20 autosomal STR loci in a sample of 50 unrelated healthy adults from the Rabari population. Hence, there is a need to report more data in the studied population. However, to date, few studies have been reported on autosomal STRs in the Gujarat population. It is the key to obtain reliable results in an analysis of DNA profiles (Butler 2009). Allelic frequency data obtained from unrelated individual in a population is essential. The genomic characteristics such as short sequence lengths, high polymorphism, and amplifying minute quantities of template DNA make these STR useful genetic markers in forensic DNA typing (Butler 2011 Nwawuba Stanley et al. ![]() Short tandem repeats (STRs) markers have gained much popularity in forensic DNA analysis for human identity testing, paternity testing, and population genetics studies (Wyner et al. However, Fstat demonstrated better genetic software for analysis of the demographic structure of a specific or set of populations. With respect to the results, the 20 STR loci are highly polymorphic and discriminating in the Gujarat population and could be used for forensic practice and population genetics studies. The combined match probability (CPM) for all 20 loci was 2.5 × 10 −22. Both the combined power of discrimination (CPD) and the combined power of exclusion (CPE) for the 20 analyzed loci were higher than 0.999999. Based on heterozygosity and the polymorphism information content, FGA may be considered as the most informative markers. The highest allele frequency detected was 0.480 for allele 9 at locus TH01. ResultsĪ total of 170 alleles were observed in the Rabari Tribe of Gujarat population, and allele frequencies ranged from 0.010 to 0.480. DNA was extracted using the organic extraction method, 20 autosomal STR loci were amplified using PowerPlex 21 kit (Promega, Madison, WI, USA) and detected on 3100 Genetic Analyser (Life Technologies Corporation, Carlsbad, CA, USA). The comparison is performed on the profiles obtained from fifty unrelated blood samples of healthy male individuals. ![]() Three statistical software packages named Cervus, Genepop, and Fstat are compared and contrasted. The present paper aims to provide a practical guide to the analysis of population genetics data. These statistics deal with the measurement of uncertainty and also provides a probability of a random match. Numerous bio-statistical software programs are available for the interpretation of population data in forensics. The present study aimed to investigate the genomic diversity in Gujarat’s Rabari population, using 20 autosomal genetic markers. The use of high-throughput molecular marker technologies and advanced statistical and software tools have improved the accuracy of human genetic diversity analysis in many populations with limited time and resources. Today, when forensic experts talk about quantifiable hereditary traits, they do not just depend on the assessment and examination of DNA profiles but also relate them to the population structures. ![]()
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